Pmscv-loxp-dsred-loxp-egfp-puro-wpre
WebKEYWORDS pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8467) AUTHORS Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, Clevers H TITLE Controlled gene expression in primary Lgr5 organoid cultures. JOURNAL Nat Methods. 2011 Dec 4. … WebMar 2, 2024 · pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE 载体 质粒类型: Cre/Lox系统，逆转录病毒载体 载体大小: 8500 bp (查看载体序列) 载体抗性: Ampicillin 备注: Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression. 产品编号 产品名称 规格 …
Pmscv-loxp-dsred-loxp-egfp-puro-wpre
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WebStable Jurkat floxRG cells were made by retroviral transduction of pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE packaged with pCL-10A1 retrovirus packaging vector. Viral … WebAddgene inc pmscv loxp dsred loxp egfp puro wpre Pmscv Loxp Dsred Loxp Egfp Puro Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
WebMay 15, 2024 · The GC AZ521 cells were transfected with pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE plasmids and cocultured with exosomes derived from GCFs transfected with Cre-expressing plasmids for 72 hours. Under the BX51 Fluorescence Microscope BX51TF (Olympus; Shinjuku, Tokyo, Japan), the successful internalization of exosomes by GC cells … WebJun 7, 2024 · Two lentiviral plasmids LV-Thy1.2-Cre pLKO.1 and pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE were used in our cardiac fibroblast lineage-tracing experiments. The …
WebpMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE是由北京索莱宝科技有限公司代理或销售的Solarbio品牌的试剂，产品来源于北京 。北京索莱宝科技有限公司是中国最权威 …
WebJul 30, 2016 · GFP-positive cells were sorted and used to isolate EVs with mCherry on the surface. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE was used as above to produce viral particles that were then used to infect BDEneu cells. Puromycin was … rusk software volumizing shine lotionWebpMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Information. Promoter: PGK. Replicon: pUC ori. Plasmid classification: virus series, reverse virus cloning cloning vector. Plasmid size: … rusk shampoo for gray hairWebGFP-positive cells were sorted and used to isolate EVs with mCherry on the surface. pMSCV-loxp-dsRed- loxp-eGFP-Puro-WPRE was used as above to pro- duce viral particles that were then used to infect BDE- neu cells. Puromycin was used to select for stably infected cells. schaum\u0027s outline of french grammar 7eWebrecognition sites, two loxP sites, each consisting of 34 bp, flank the targeted site called a “floxed allele”. Cre-mediated recombination causes fusion of two flanked loxP sites and releases this DNA part [17]. Furthermore, a floxed reporter gene such as β-galactosidase or a fluorescent protein in the cell visually shows the effect of Cre ... rusk sheriff\u0027s officeWebAug 26, 2024 · Although pH-dependent membrane active peptides (PMAPs) are employed to disrupt the endosomal membrane, the efficiency of endosomal escape remains low 10. This outcome may be due to the interaction... rusks baby cerealWeb质粒载体网zlzt.com专业提供pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE,产品编号为zl-016233,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完 … rusk speed freak warrantyWebSep 24, 2024 · In our studies, we isolated GFP+ and DsRed+ cells from primary orthotopic mouse tumors and used them to generate spheroids containing 10–20% tumor-derived cells and 80–90% unlabeled type cells. Using live cell imaging, we tracked individual cells migrating within the spheroid as well as invading into the adjacent collagen matrix ( … rusk speed freak blow dryer ctc lite